1.教育状况

2013.09-2017.06：东北农业大学动物营养与饲料科学专业，博士

2015.01-2017.01：加拿大曼尼托巴大学化学专业，联合培养博士

2010.09-2013.06：东北农业大学动物营养与饲料科学专业，硕士

2006.09-2010.06：东北农业大学动物科学专业，学士

2.工作经历

2025.09-至今：东北农业大学动物科学技术学院，教授，博士研究生导师

2020.09-2025.08：东北农业大学动物科学技术学院，副教授

2018.08-2023.04：东北农业大学生物学博士后流动站，博士后

2017.10-2020.08：东北农业大学动物科学技术学院，讲师

代表性论文（第一作者或通讯作者）：

[1] Yinfeng Lyu#, Meng Xue#, Xinyue Huang, Yifeng Bian, Peiyang Li, Chengyi Yang, Licong Zhang, and Anshan Shan*. Peptide Dendrimers: Novel Therapeutic Opportunities for Peptide-Based Biomaterials in Biomedicine. Journal of Controlled Release, 2025, 388: 114322. (IF: 11.5，中科院一区TOP)

[2] Xu Guo#, Weikang Yu#, Qingrui Li, Xuefeng Li, Yingxin Wei, Jiajun Wang, Yinfeng Lyu* and Anshan Shan*. Overcoming Clinical Barriers: Utilizing Self-Assembly Systems to Break the Protease Limitations of Antimicrobial Peptides. Aggregate, 2025, 0: e70193. (IF: 13.7，中科院一区TOP)

[3] Xi Yan#, Yinfeng Lyu#, Yi Liu, Jianping Ren, Yue Zhao, Licong Zhang, Anshan Shan*. Utilizing Proteolytic-Resistant Nano-Short Peptide Based on Naphthyl Tail-Anchored to Combat Bacterial Infections. Advanced Science, 2025, e08854. (IF: 14.1，中科院一区TOP)

[4] Guoyu Li#, Hongyu Chen#, Wenwen Chen, Zhenheng Lai, Yinfeng Lyu* and Anshan Shan*. Self-assembled nanopeptide dendrites with high antifungal activity and protease hydrolytic stability for fungal keratitis treatment. Journal of Nanobiotechnology, 2025, 23: 577. (IF: 12.6，中科院一区TOP)

[5] Weikang Yu, Mengyi Zhao, Xu Guo, Xiangwan Wang, Jiajun Wang*, Yinfeng Lyu*, Anshan Shan*. Proteolytic-resistant self-assembling peptide nanofibers combat specific bacterial infections via trap and kill. Science Advances, 2025, 11(29), eadx0153. (IF: 12.5，中科院一区TOP)

[6] Yinfeng Lyu, Peiyang Li, Yifeng Bian, Wenjing Hou, Bo Li, Licong Zhang, Anshan Shan. Antimicrobial peptide-chitosan nanoparticles combat ETEC-induced bacterial infection in mice. International Journal of Biological Macromolecules, 2025, 311(Pt4): 144047. (IF: 8.5，中科院二区TOP)

[7] Yinfeng Lyu#, Yang Yang#, Peiyang Li, Chuqiao Zhou, Licong Zhang, Chongpeng Bi, Anshan Shan*. The Effects of Antimicrobial Peptide-Chitosan Nanoparticles on Alleviating Ulcerative Colitis in Citrobacter rodentium Infected Mice. Food Bioscience, 2025, 68: 106575. (IF: 5.9，中科院二区TOP)

[8] Xi Yan, Chengyi Yang, Bo Li, Yifeng Bian, Weikang Yu, Yongjie Zhu, Baojing Cheng, Yinfeng Lyu*, Anshan Shan*. Positive Charge-Concentrated Dimeric Lipopeptides with Enhanced Protease Resistance: A Potential Solution for Systemic Bacterial Infections. Journal of Medicinal Chemistry, 2025, 68(2):1397-1416. (IF: 6.8，中科院一区TOP)

[9] Zhenheng Lai#, Xiaojie Yuan#, Wenwen Chen, Hongyu Chen, Bowen Li, Zhongpeng Bi, Yinfeng Lyu* and Anshan Shan*. Design of Proteolytic-Resistant Antifungal Peptides by Utilizing Minimum D‑Amino Acid Ratios. Journal of Medicinal Chemistry, 2024, 67, 10891-10905. (IF: 6.8，中科院一区TOP)

[10] Yinfeng Lyu, Meishu Tan, Meng Xue, Wenjing Hou, Chengyi Yang, Anshan Shan*, Wensheng Xiang, Baojing Cheng. Broad-spectrum Hybrid Antimicrobial Peptides Derived from PMAP-23 with Potential LPS Binding Ability. Biochemical Pharmacology, 2023, 210: 115500. (IF: 5.6，中科院二区)

[11] Tingting Chen#, Yinfeng Lyu#, Meishu Tan, Chengyi Yang, Ying Li, Changxuan Shao, Yongjie Zhu, and Anshan Shan*. Fabrication of Supramolecular Antibacterial Nanofibers with Membrane-Disruptive Mechanism. Journal of Medicinal Chemistry, 2021, 64(22): 16480–16496. (IF: 6.8，中科院一区TOP)

[12] Jiawei Li#, Lu Shang#, Jing Lan, Shuli Chou, Xingjun Feng, Baoming Shi, Jiajun Wang, Yinfeng Lyu*, Anshan Shan*. Targeted and intracellular antibacterial activity against S. agalactiae of the chimeric peptides based on pheromone and cell-penetrating peptides. ACS Applied Materials & Interfaces, 2020,12(40): 44459-44474. (IF: 8.2，中科院二区)

[13] Yinfeng Lyu, Chengyi Yang, Tingting Chen, Lu Shang, Yang Yang, Jiawei Li, Anshan Shan*, Wensheng Xiang, Baojing Cheng and Licong Zhang. Characterization of an antibacterial dodecapeptide from pig as a potential food preservative and its antibacterial mechanism. Food & Function, 2020, 11(5): 4090-4102. (IF: 5.4，中科院二区TOP)

[14] Yinfeng Lyu, Tingting Chen, Lu Shang, Yang Yang, Zhongyu Li, Jiang Zhu, and Anshan Shan*. Design of Trp-rich dodecapeptides with broad-spectrum antimicrobial potency and membrane-disruptive mechanism. Journal of Medicinal Chemistry, 2019, 62(15): 6941-6957. (IF: 6.8，中科院一区TOP)

[15] Yinfeng Lyu, Ronald Domalaon, Xuan Yang, Frank Schweizer*. Amphiphilic lysine conjugated to tobramycin synergizes legacy antibiotics against wild-type and multidrug-resistant Pseudomonas aeruginosa. Peptide Science, 2019, 111(1): e23091. (IF: 3.2，中科院四区)

[16] Yinfeng Lyu, Xuan Yang, Sudeep Goswami, Bala Kishan Gorityala, Temilolu Idowu, Ronald Domalaon, George G. Zhanel, Anshan Shan*, Frank Schweizer*. Amphiphilic tobramycin-lysine conjugates sensitize multidrug resistant Gram-negative bacteria to rifampicin and minocycline. Journal of Medicinal Chemistry, 2017, 60(9): 3684-3702. (IF: 6.8，中科院一区TOP)

[17] Yinfeng Lyu, Yang Yang, Xiting Lyu, Na Dong, Anshan Shan*. Antimicrobial activity, improved cell selectivity and mode of action of short PMAP-36-derived peptides against bacteria and Candida. Scientific Reports, 2016, 6:27258. (IF: 3.9，中科院三区)

[18] Yinfeng Lyu, Jiajun Wang, He Gao, Zeyun Wang, Na Dong, Qingquan Ma, Anshan Shan*. Antimicrobial properties and membrane-active mechanism of a potential α-helical antimicrobial derived from cathelicidin PMAP-36. PLoS One, 2014, 9: e86364. (IF: 2.6，中科院三区)

[19] 吕银凤，薛萌，黄欣月，谭美姝，侯文婧，单安山*. 抗菌肽联合传统抗菌药物对抗细菌耐药性研究进展. 中国感染控制杂志. 2023, (22)10：1266-1273.

[20] 吕银凤，白金妮，谭德智，陈婷婷，单安山. 铰链结构对抗菌肽生物活性的影响及在分子设计中的应用研究进展. 生物工程学报，2021, 37(9): 3142-3150.

授权专利

[1] 吕银凤等；一种β-折叠抗菌肽HINGE-RV及其制备方法和应用；ZL202110777078.4

[2] 吕银凤等；一种靶向革兰氏阴性菌的富脯氨酸抗菌肽Pyr-2及其制备方法与应用；ZL202110776781.3

[3] 吕银凤等；一种超短自组装抗菌肽FWR及其制备方法与应用；ZL202210372565.7

[4] 吕银凤等；一种富含Trp的α-螺旋结构抗菌肽W33及其制备方法和应用；ZL202310714576.3

[5] 吕银凤等；富含非天然氨基酸的树状支链抗菌肽及其制备方法和应用；ZL202410296894.7

[6] 吕银凤等；富含D-Arg的抗酶解十四肽2rrr及其制备方法和应用；ZL202410971350.6

[7] 吕银凤等；一种靶向革兰氏阴性菌抗菌肽及其壳聚糖纳米微粒和应用；ZL202410775647.5

[8] 吕银凤等；一种具有高蛋白酶稳定性的抗菌短肽及其制备方法和应用；ZL202411338223.9

[9] 吕银凤等；一种分支结构抗菌肽KW及其制备方法和应用；ZL202311267555.8

[10] 吕银凤等；一种PEG偶联树枝状分支抗菌肽及其制备方法和应用；ZL202311390380.X

在研课题

1. 国家自然科学基金面上项目，群体感应系统介导抗菌肽-肉桂醛协同抑制细菌毒力及肠道定植机制研究，2025.01-2028.12，主持

2. 国家重点研发计划项目子课题，新型抗酶解抗菌肽和免疫调节肽的创制及其抗感染机制，2023.12-2028.11，主持

3. 国家自然科学基金青年基金项目，LPS靶向抗菌肽-壳聚糖纳米微粒抑制断奶仔猪肠道炎症反应研究，2020.01-2022.12，主持

4. 黑龙江省自然科学基金优秀青年项目，非完美α-螺旋结构抗菌肽的构效关系及抗菌机制研究， 2019.07-2022.07，主持

5. 中国博士后科学基金特别资助项目，抗菌肽与黄芩苷的协同抗菌活性及机制研究，2022.07-2023.08，主持

6. 中国博士后科学基金面上资助项目，铰链影响α-螺旋抗菌肽生物学活性及抑菌机制的研究，2019.01-2023.08，主持

7. 黑龙江省普通本科高等学校青年创新人才培养计划，高效靶向抗菌肽的分子设计、生物学活性及作用机理研究，2017.11-2020.10，主持

8. 黑龙江省博士后特别资助项目，自组装β折叠抗菌肽的分子设计、构效优化及抗菌机制研究，2022.01-2023.08，主持

9. 黑龙江省博士后面上资助项目，含铰链结构抗菌肽的构效关系及抑菌机理研究，2019.01-2023.08，主持

10. 东北农业大学“东农学者计划”-“学术骨干”项目，基于Fmoc基团的自组装抗菌肽分子设计及生物学活性研究，2022.01-2024.12，主持

11. 纳米抗菌肽的构建及生物学活性研究，东北农业大学“东农学者计划”-“青年才俊”项目，2018.11-2021.11，主持

12. 农业部东北动物营养与饲料科学观测试验站开放课题，不同作用靶点抗菌肽的构效优化及联合应用的协同抗菌活性研究，2018.01-2019.12，主持